Cloning,Expression and Bioinformatics Analysis of Enterococcus faecalis from Northeast Black Bear

In order to detect Enterococcus faecalis endocarditis antigen (EfaA) of bear that was used to disclose the infected Northeast Black bear immediately, a pair of PCR primers was synthesized by EfaA gene of Enterococcus faecalis that was recorded in GenBank (accession number:U03756.1) and the target fragment was 689 bp. The PCR product was cloned by pMD19-T vector, then was transferred to Escherichia coli DH5α competent cells and the positive clones were filtered. Recombinant plasmid (pMD19-T-EfaA) was extracted. Identification of PCR and digestion, sequencing, the structure prediction of proteins were operated. The results showed that EfaA gene was cloned successfully, and the gene homology with ATCC 29212 was 100.0%. pMD19-T-EfaA was constructed successfully, structure of proteins was predicted. This study provided theoretical basis for diagnostic methods of Enterococcus faecalis and laid basis for prevention and control of Northeast Black bear disease.     

黑杨派南方型无性系高生长与气候条件的斜率关联度分析

用灰色系统理论的斜率关联度分析法，求得影响黑杨派南方型无性系高生长的主要气候因子依次为：降水量、极低温、极高温、蒸发量、日照时数、积温、年均温（或相至湿度）、相以湿度（或年均温）、无霜期。     

Differences in the behavior of the crossbred steers of different sire lines of Japanese Black cattle

We determined differences in the behavior of the progeny of two major sire lines of Japanese Black cattle by recording the behavior of 35 and 70 half-sib steers of sires from fast (FG) and slow (SG) growing lines, respectively. Two sire lines of steers were mixed and allocated to nine pens with 11–12 animals per pen. The proportion of steers lying was significantly (P < 0.001) higher in the SG line (43.4 ± 5.7% compared to 40.3 ± 6.0%). The proportion of time spent eating concentrate feed (FG: 12.1 ± 2.3%; SG: 11.4 ± 2.1%), drinking (FG: 0.8 ± 1.1%; SG: 0.4 ± 0.6%), licking the feed trough (FG: 0.4 ± 0.6%; SG: 0.2 ± 0.4%) and performing tongue-playing (FG: 3.1 ± 4.6%; SG: 1.0 ± 1.9%) was significantly higher in FG, whereas the proportion of time spent resting (FG: 41.5 ± 12.8%; SG: 43.7 ± 10.9%) and performing self-licking (FG: 1.7 ± 1.4%; SG: 2.1 ± 1.3%) was higher in SG (all P < 0.05). These results show progeny of the FG sire engaged in more active behaviors compared to the progeny of the SG sire line.     

Fengshui forest management by the Buyi ethnic minority in China

Fengshui forests, very popular among the ethnic minorities in the rural areas in Guizhou Province of China, have spiritual symbolic meanings and are strictly managed. This paper examines the nature, history, local perceptions and management of fengshui forests in two Buyi villages in the Guizhou Province. With their long history, the fengshui forests are perceived to provide good wishes, security, unity, fortune, wealth and health to the local communities. Fengshui forests act as a form of “community based nature reserve” which maintains the health of natural forests, especially hydrological features and prevention of soil erosion. Cunguimingyue (Folk Regulation and Customs) is the primary local normative mechanism that maintains the fengshui forests in a good condition. The lessons learnt from local fengshui forest management needs to be incorporated in for contemporary China's forest policy formulation as these forests play an important role in rural livelihoods.     

贵州省农业产业结构研究

运用产业结构相似系数、产业结构变化系数和描述产业结构稳定性的熵来对贵州省农业产业结构现状进行分析与评价,其研究成果对确定贵州省农业产业结构调整方向和发展贵州省农业农村经济有一定的参考价值。     

贵州屯堡文化在现代园林景观中的应用——以平坝县高烟坡山体公园规划设计为例

指出了屯堡文化作为贵州独特的汉族文化,其截然迥异的民族风格为现代园林地域景观提供了多元化参考。通过以平坝县高烟坡山体公园景观设计为例,对屯堡文化景观元素进行了提取,以促进现代园林景观设计的地域化、乡土化,保护和传承民族文化。     

黄连、黄芩提取液与二氧化氯复配对结球甘蓝种传黑斑病的抑菌效果研究

采用三元二次回归正交旋转组合设计方法,确立了黄连、黄芩提取液与二氧化氯对甘蓝链格孢菌抑制效果最佳的复配组合:黄连提取液13.48%、黄芩提取液15.56%、二氧化氯0.14%.用此复配剂浸种,芸苔链格孢菌和甘蓝链格孢菌的侵染率分别降低了97.33%和94.85%,且提高了种子发芽势及萌发相关酶活性.     

Phytochemical and antioxidant properties of anthocyanin-rich Morus nigra and Morus rubra fruits

In this study, phytochemical and antioxidant properties of anthocyanin-rich mulberry species of Morus nigra L. (black mulberry) and Morus rubra L. (red mulberry) fruits harvested from across Turkey were investigated. Fruit color, total phenolics (TP), total monomeric anthocyanin (TMA), titratable acidity (TA), and individual sugar and organic acid compositions were determined. Total antioxidant capacity (TAC) of fruits was assessed by both the trolox-equivalent antioxidant capacity (TEAC) and the ferric reducing antioxidant power (FRAP) assays. Black mulberry exhibited higher TP, TMA, TAC and TA when compared to red mulberry. The average TP contents of M. nigra and M. rubra were 2737 and 1603 μg gallic acid equivalent in g fresh weight basis (GAE/g fw), respectively. M. nigra had the richest amount of anthocyanin with an average of 571 μg cy-3-glu/g fw. Overall, TAC averaged 10.5 and 12.0 mmol TE/L by the TEAC and FRAP methods, respectively. We found that FRAP, TEAC, TP and TMA were significantly correlated (r = 0.64–0.99) with each other. Fructose (5.27 g/100 mL) and glucose (5.81 g/100 mL) were determined to be the major sugars in both mulberries. M. nigra displayed a higher TA (2.05 g/100 mL) than M. rubra (0.78 g/100 mL), with citric acid as the major acid.     

安哥拉山羊级进杂交后代生活力初步观察

对安哥拉山羊及其级进杂交后代的生长发育、繁殖力、抗病性、采食习性等进行初步观察研究，结果表明：在试验场良好的饲养条件下，各代杂种羊生长发育正常，但繁殖力、抗病性、采食能力随级进杂交代数的增加有降低的趋势。据此，笔者认为，在陕北特定的生态经济条件下，培育毛用山羊新品种，级进杂交代数不宜太高，到Ｆ３代应停止级进，进行横交固定，而且在整个育种中，应始终注意饲养问题，以保证新品种培育成功。     

黑小麦新品种的创育及其应用策略探讨

本文简要分析了当前黑小麦品种的特点，根据实践对黑小麦新品种的创育提出了不同的目标和途径，介绍了2个黑小麦新品种并对其应用的策略进行了探讨。